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Biology

Cell Bio chapter 8

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Concept 8.1 What is energy? Potential energy = stored energy Chemical bonds Concentration gradients (can be used to do cellular work) Electrical potential Kinetic energy = movement energy Heat = molecular motion Mechanical = moving molecules past each other Electrical = moving charged particles Forms of energy Biker at top of hill potential Biker going down hill kinetic Why do cells need energy? Synthetic work = building macromolecules (e.g., making protein) mechanical work = moving molecules past each other e.g., muscle shortening concentration work = creating chemical gradients e.g. storing glucose electrical work = creating ion gradients e.g., unequal distribution of sodium and potassium ions Metabolic pathways Catabolic pathways ? release energy

Ecology: background information

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Ecology: Definitions Ecology: The scientific study of the interactions between organisms and their environment that determines the distribution and abundance of organisms Ecology is a science Environmentalism is a cause Conservation Biology is the integration of these two: using science to support a political cause Population ecology: experimental field approach: natural populations manipulated to test specific predictions arising from controversial ecological theory Organismal ecology: studies how an organism?s structure, physiology, and (for animals) behaviour meet environmental challenges Population: all the individuals of the same species within an ecosystem Population ecology focuses on factors affecting how many individuals of a species live in an area

Chromatin folding

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Chromatin: The formation of chromosomes Chromatin is packed into chromosomes during mitosis. We will look at the steps from interphase to metaphase There are several levels of organisation in terms of chromatin folding? Free DNA 2nm Formation of the nucleosome: The DNA strand wraps around a core of eight histones (H2A, H2B, H3, H4: x2) 1.65 times. Also known as ?beads-on-a-string? and is the basic chromatin structure. 10nm An H1 histone tails binds on the outside of the coil on each nucleosome unit, now chromatosome units. Further condensing occurs: 30nm Interactions between: Interactions between chromatosomes (nucleosomes and H1 histones) leads to more folding of the 30nm fibres, creating a thicker fibre. 30nm

Regulation of the cell cycle

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Cell Cycle Regulation Cell cycle control systems The frequency of cell division varies with the type of cell Caused by the difference in regulation at the molecular level The stages of the cell cycle are regulated by a distinct cell cycle control system (similar to a clock) The cell cycle control system is regulated by both internal and external controls The clock has specific checkpoints where the cell cycle stops until a go-ahead signal is received Cell cycle control systems: interphase and mitosis Interphase Makes up about 90% of the cell cycle: can be divided into subphases: G1 phase (?first gap?) S phase (?synthesis?) G2 phase (?second gap?) The cell grows during all three phases Chromosomes are only duplicated during the S phase

Cell Membranes

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Cell Membranes Contents: Structure: the basics Fluid mosaic model/freeze fracture technique Membrane fluidity Membrane fluidity and cholesterol The Basics? The plasma membrane separates the living cell from its surroundings Phospholipids make up the majority of the bilayer Phospholipids consists of a hydrophilic phosphate head and two hydrophobic fatty acid tails Phospholipids are amphipathic molecules: containing both a hydrophilic and hydrophobic region The plasma membrane is described by the fluid mosaic model: The membrane is fluid with a variety of ?mosaic? proteins embedded in it The plasma membrane is selectively permeable: Small fat soluble (hydrophobic ? non polar) molecules, can enter the cell through the lipid bilayer (this occurs rapidly) e.g. hydrocarbons

The Cytoskeleton

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The Cytoskeleton The cytoskeleton extends throughout the cytoplasm It organises cell structure and activities, anchoring many organelles in place It provides mechanical support, maintaining the cells shape: it?s domed in shape and stabilised by opposing forces It is also very dynamic, and can be dismantled and reassembled quickly, to change the shape of the cell Form The cytoskeleton is a network of fibres, the molecular structures include: Microtubules: the thickest of the fibres at 25nm (15nm lumen) Microfilaments: the thinnest of the fibres at 7nm Intermediate filaments: range in diameter from 8?12nm, larger than microfilaments but smaller than microtubules Roles

The Endomembrane system

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The Endomembrane system Organelle recap? Ribosomes Ribosomes: a complex of ribosomal RNA (rRNA, synthesised in the nucleolus) and proteins Ribosomes carry out protein synthesis in: The cystol/plasm (free ribosomes) On the outside of the rough endoplasmic reticulum Free ribosomes make proteins used within the cell e.g. enzymes Bound ribosomes make proteins which are often secreted e.g insulin made in pancreatic cells Endoplasmic reticulum Form Accounts for more than half of the total membrane in many eukaryotic cells The ER membrane is continuous with the nuclear envelope There are two distinct regions of ER: Smooth ER: lacks ribosomes Rough ER: ribosomes are bound to the outer membrane Functions: Smooth endoplasmic reticulum

Types of chromatin

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Chromatin: Types of chromatin Chromatin In non-dividing cells, the DNA exists as chromatin and appears uncoiled and randomly dispersed After DNA replication (S phase) the chromatin begins to condense in preparation for mitosis Chromatin consists of 50% DNA, 50% proteins, mainly histones Chromatin is the structure that the eukaryotic genome is packaged into It allows long molecules of DNA to fit into the small volume of the nucleus The basic structural unit of chromatin is the nucleosome Further folding and compaction of chromatin produces visible metaphase chromosomes during cell division Heterochromatin vs Euchromatin Euchromation Active Chromatin The majority of chromatin is this form Less condensed, lightly packed in the nucleus (basic ?beads on a string? form ?check)

The Nucleus

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The Nucleus Functions of the nucleus Storage of genes on chromosomes Transportation of regulatory factors & gene products via nuclear pores Separation of: DNA from cytosol Transcription from translation Organisation of: genes into chromosomes (to allow cell division) uncoiling of DNA to replicate key genes Production of: messenger RNA ( mRNA ) that code for proteins ribosomal RNA (rRNA) in the nucleolus Components within the nucleus Nuclear envelope: double membrane, each membrane is a phospholipid bilayer Nuclear pore: controls entry and exit from the nucleus e.g. of RNA proteins Nuclear lamina: a series of structural proteins found on the inner membrane of the nuclear envelope, provide structural support Nuclear matrix: scaffold proteins Nucleoplasm: viscous medium

Speciation

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The last ice age produced many different species mainly because of vicariance events; the glaciers physically separated populations from each other. Sympatry: populations that live close enough to interbreed Gene flow prevents speciation in sympatric populations A mating between a tetraploid individual and a diploid individual produces: triploid individuals: which likely produce nonviable gametes with an uneven number of chromosomes All polyploid individuals contain more than two haploid sets of chromosomes
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