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RNA

Gene Regulation powerpoint

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Chapter 18: Regulation of Gene Expression Correlations to AP Bio Course Curriculum See Unit 3 Essential Knowledge document for all EK statements for Chapter 18. Correlations to AP Bio Course Curriculum Learning Objective 3.18: The student is able to describe the connection between the regulation of gene expression and observed differences between different kinds of organisms. Learning Objective 3.19: The student is able to describe the connection between the regulation of gene expression and observed differences between individuals in a population. Correlations to AP Bio Course Curriculum Learning Objective 3.20: The student is able to explain how the regulation of gene expression is essential for the processes and structures that support efficient cell function.

Macromolecules review

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Chapter 4: Carbon and the Molecular Diversity of Life 4 Major Macromolecules: Lipids Carbohydrates Nucleic Acid Protein Lipids Fat- a combination of a glycerol and three fatty acids also called a triglyceride Used for long term energy storage, which is located in the long carbon chains Phospholipid- a combination of a glycerol, two fatty acids and a phosphate group Makes up cell membrane (lipid bilayer) Selectively permeable Hydrophilic heads (the phosphate group) Hydrophobic tails Steroid- lipids with a structure of four fused rings General Information Glycerol is combined to fatty acid by an ester linkage (dehydration synthesis) Ester linkage is polar CH2 groups are nonpolar Saturated Fat- a triglyceride with only single bonds

DNA and Genes

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Protein biosynthesis

Molecular biology

Gene expression

Nucleic acid sequence

DNA and Genes (Extended Notes) DNA: The Molecule of Heredity What is DNA? An organism?s environment influences how an organism develops, but the organism?s DNA holds the genetic information and determines its traits. DNA achieves this by controlling the structure of proteins. Your body is made up of proteins Your body?s functions depend on proteins called enzymes In the 1950s scientists thought protein was the genetic material In 1952 Hershey and Chase infected bacteria with radioactive viruses (one was radioactive protein & the other was radioactive DNA) Only the radioactive DNA entered the bacteria and produced new viruses DNA is a complex organic molecule called a polymer. The monomers, or repeating subunits, that make up DNA are called nucleotides.

Campbell Biology 9th Edition Chapters 14-18 Study Guide

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Gene expression

Protein biosynthesis

Molecular biology

Chapters 14 and 15 Gregor Mendel Studied traits that occur in distinct forms Developed true-breeding varieties When bred amongst themselves, by self-fertilization, these plants produced offspring identical to the parent for that trait No variation in that one trait Used mathematical analysis in his studies Findings related mainly to plants, not humans Definitions Gametes: reproductive cells produced by sexually-reproducing organisms Two types: Male gametes=sperm In plants: contained in pollen Female gametes=eggs In plants, contained in ovules, which mature to seeds when fertilized Ovules contained in carpels Fertilization Fertilization: fusion of egg and sperm Self-fertilized: fusion of sperm and egg from same plant

DNA, RNA, and Protein Synthesis

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Gene expression

Protein biosynthesis

DNA, RNA, and Protein Synthesis DNA -The species-particular DNA sequences produce the species-particular proteins -Genes code for proteins -Genes are long strands of DNA of chromosomes WHAT IS DNA? -DNA is genetic code -Instructions for heredity -Components of genes -Director of protein synthesis -A type of nucleic acid -A type of organic compound -A polymer {a compound made of repeating subunits} DNA'S PROPER NAME IS -Deoxyribonucleic acid -Consists of a ribose sugar with a "missing oxygen" (thats the de-oxy part) -And it's found in the nucleus of eukaryotic cell STRUCTURE OF DNA -A nucleotide of DNA is the base unit -A nucleotide consists of a phosphate, a sugar, and a nitrogen base DNA IS A DOUBLE STRAND -The nitrogen bases have compliment partners

Bio_160_Lecture4

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Gene expression

Molecular biology

Molecular genetics

Transcription factor

Regulation of gene expression

Gene Expression Chapter 11 ? Differentiation yields a variety of cell types, each expressing a different combination of genes ? Different types of cells make different proteins because different combinations of genes are active in each type Muscle cell Pancreas cells Blood cells Fiure 11.2 Differentiated cells may retain all of their genetic potential Most differentiated cells retain a complete set of genes Root of carrot plant Root cells cultured in nutrient medium Cell division in culture Plantlet Adult Plant Single cell Figure 11.3 DNA packing in eukaryotic chromosomes helps regulate gene expression ? A chromosome contains DNA wound around clusters of histone proteins ? This beaded fiber is further wound and folded

AP Biology Chapter 17 Notes Campbell/Reece

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Protein biosynthesis

Gene expression

Prokaryotic translation

AP Biology Chapter 17 (17.3 and 17.4) 17.3 Eukaryotic cells modify RNA after transcription RNA Processing: in the nucleus ? before it is sent out to the cytoplasm. both ends of the primary transcript are altered certain interior sections are cut out A. Alternation of mRNA Ends (Figure 17.9) Both ends of the mRNA are modified in a particular way 5prime end ? 5prime cap made of modified form of a Guanine nucleotide 3prime end ? poly A tail - 50 to 250 Adenine (A) nucleotides are added. Remember, the mRNA is released soon after the signal AAUAAA is transcribed. So even more A is added on after that. Functions of 5prime cap and poly A tail: facilitate export of the mature mRNA from the nucleus protect the mRNA from degradation by hydrolytic enzymes.

AP Biology Chapter 16 Notes Campbell/Reece

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Molecular biology

DNA Replication

Molecular genetics

Okazaki fragments

Nucleotide excision repair

Chapter 16 The Molecular Basis of Inheritance Essay questions will be on Feulgen, Griffith experiement, Hershey and Chase, Chargoff, Watson and Crick, Tatum Beadle Experiment Overview: Your genetic material is contained in: 1. 46 chromsomes from mom and dad 2. Mitochondrial DNA from mom Nucleic acids - Unique from all other molecules in that they can direct their own replication. 16.1 DNA is the genetic material A. The Search for Genetic Material: Scientific Inquiry Initially, Genes were noted to be on chromosomes (b/c of work by Morgan an d Mendel) ? so people didn?t know if they were on the proteins or on the DNA. 1. Frederick Griffith (figure 16.2)? Showed evidence that DNA (not protein) can transform bacteria Background:

ps 2.3

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Gene expression

Molecular biology

Protein biosynthesis

Nucleic acid sequence

Practice Problems for Molecular Biology, Session 3: Transcription, Translation ? ? Question 1 ? ? Fill in the table: ? Transcription Translation Where does this process occur in a eukaryotic cell? ? ? What is the enzyme that carries out this process? ? ? What is the template that is read during this process? ? ? In what direction is the template read? ? ? What is the start signal/sequence for this process? ? ? What is the polymer that is formed? ? ? What monomer is used to form this polymer? ? ? What type of bond is formed between monomers? ? ? In what direction is the new polymer formed? ? ? What is the stop signal/sequence for this process? ? ?

exam 2

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Gene expression

Molecular biology

Reverse transcriptase

DNA Replication

Molecular Biology Unit Exam Question 1 Consider the following origin of replication that is found on a chromosome. The sequence of region 1 is shown below. Region 1: 5??CTGACTGACA?3? 3??GACTGACTGT?5? 3? 5? 5? 3? Region 1 top ? ori bottom ? a) Within Region 1, which strand will be the template for leading strand synthesis, the top or the bottom? b) If we assume that a lagging strand fragment is made from region 1, what will be its sequence? c) You examine DNA replication in an E. coli mutant, which has a partially defective DNA polymerase. In vitro experiments using the mutant DNA polymerase gives an error rate of 10-3, as compared to the expected error

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