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AP Biology Chapter 16 Notes Campbell/Reece

Subject: 
Biology
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Biology
Genetics
Molecular biology
DNA Replication
Molecular genetics
DNA
Gene
Okazaki fragments
Chromatin
Transcription
RNA
Nucleotide excision repair
health

Chapter 16 The Molecular Basis of Inheritance Essay questions will be on Feulgen, Griffith experiement, Hershey and Chase, Chargoff, Watson and Crick, Tatum Beadle Experiment Overview: Your genetic material is contained in: 1. 46 chromsomes from mom and dad 2. Mitochondrial DNA from mom Nucleic acids - Unique from all other molecules in that they can direct their own replication. 16.1 DNA is the genetic material A. The Search for Genetic Material: Scientific Inquiry Initially, Genes were noted to be on chromosomes (b/c of work by Morgan an d Mendel) ? so people didn?t know if they were on the proteins or on the DNA. 1. Frederick Griffith (figure 16.2)? Showed evidence that DNA (not protein) can transform bacteria Background:

ps 2.3

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Gene expression
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RNA
Protein biosynthesis
Translation
Nucleic acid sequence
Messenger RNA
Gene
DNA
Transcription

Practice Problems for Molecular Biology, Session 3: Transcription, Translation ? ? Question 1 ? ? Fill in the table: ? Transcription Translation Where does this process occur in a eukaryotic cell? ? ? What is the enzyme that carries out this process? ? ? What is the template that is read during this process? ? ? In what direction is the template read? ? ? What is the start signal/sequence for this process? ? ? What is the polymer that is formed? ? ? What monomer is used to form this polymer? ? ? What type of bond is formed between monomers? ? ? In what direction is the new polymer formed? ? ? What is the stop signal/sequence for this process? ? ?

exam 2

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Lac operon
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Gene
Reverse transcriptase
DNA Replication
DNA
health

Molecular Biology Unit Exam Question 1 Consider the following origin of replication that is found on a chromosome. The sequence of region 1 is shown below. Region 1: 5??CTGACTGACA?3? 3??GACTGACTGT?5? 3? 5? 5? 3? Region 1 top ? ori bottom ? a) Within Region 1, which strand will be the template for leading strand synthesis, the top or the bottom? b) If we assume that a lagging strand fragment is made from region 1, what will be its sequence? c) You examine DNA replication in an E. coli mutant, which has a partially defective DNA polymerase. In vitro experiments using the mutant DNA polymerase gives an error rate of 10-3, as compared to the expected error

exam2 soln

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Biology
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Biology
Gene expression
Genetics
Molecular biology
RNA
Protein biosynthesis
Lac operon
Gene
Mutation
Transcription
DNA Replication
Translation
health

Solutions to Molecular Biology Unit Exam Question 1 Consider the following origin of replication that is found on a chromosome. The sequence of region 1 is shown below. Region 1: 5??CTGACTGACA?3? 3??GACTGACTGT?5? 5? 3? 5? 3? Region 1 ori top ? bottom ? a) Within Region 1, which strand will be the template for leading strand synthesis, the top or the bottom? The bottom strand will be the template for leading strand synthesis. b) If we assume that a lagging strand fragment is made from region 1, what will be its sequence? 5? T G T C A G T C A G 3? c) You examine DNA replication in an E. coli mutant, which has a partially defective DNA polymerase. In vitro

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