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Pearson Ch. 18 - Regulation of Gene Expression

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Ch 18 - Regulation of Gene Expression Overview: Conducting the Genetic Orchestra Prokaryotes and eukaryotes alter gene expression in response to their changing environment In multicellular eukaryotes, gene expression regulates development and is responsible for differences in cell types RNA molecules play many roles in regulating gene expression in eukaryotes Concept 18.1: Bacteria often respond to environmental change by regulating transcription metabolic control occurs on two levels cells can adjust the activity of enzymes already present feedback inhibition - presence of enzyme a shuts down synthesis of more enzyme a by inhibiting activity cells can regulate the expression of the genes encoding the enzymes Operons: The Basic Concept

ap_bio_chap_15_gene_regulation.ppt

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0 15 Regulation of Gene Expression Overview: Differential Expression of Genes Prokaryotes and eukaryotes alter gene expression in response to their changing environment Multicellular eukaryotes also develop and maintain multiple cell types Gene expression is often regulated at the transcription stage, but control at other stages is important, too Concept 15.1: Bacteria often respond to environmental change by regulating transcription Natural selection has favored bacteria that produce only the gene products needed by the cell A cell can regulate the production of enzymes by feedback inhibition or by gene regulation Gene expression in bacteria is controlled by a mechanism described as the operon model Regulation of gene expression Precursor trpE gene (a) Regulation of enzyme

Gene Regulation

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Gene Regulation in Eukaryotes The latest estimates are that a human cell, a eukaryotic cell, contains some 21,000 genes. Some of these are expressed in all cells all the time. These so-called housekeeping genes are responsible for the routine metabolic functions (e.g. respiration) common to all cells. Some are expressed as a cell enters a particular pathway of differentiation. Some are expressed all the time in only those cells that have differentiated in a particular way. For example, a plasma cell expresses continuously the genes for the antibody it synthesizes. Some are expressed only as conditions around and in the cell change. For example, the arrival of a hormone may turn on (or off) certain genes in that cell.

bio diagram

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glucose lactose operator (repressor binding site) PromoterZYA lacZ lacY lacAcrp lacI repressor active activator inactive PromoterIPromotercrp + - mRNA mRNA activator binding site polymerase RNA polymerase RNA operator (repressor binding site) PromoterZYA lacZ lacY lacAcrp lacI repressor active repressor lactose inactive activator inactive cAMP activator active PromoterIPromotercrp mRNA mRNA mRNA LacZ (?gal) LacY LacA activator binding site polymerase RNA polymerase RNA repressor active can metabolize lactose!cAMP + - + lactose + cAMP activator active polymerase RNA DNA DNA The lac operon: a model of gene regulation in E. coli MIT OpenCourseWare http://ocw.mit.edu 7.01SC Fundamentals of Biology Fall 2011
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