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DNA replication

AP Bio_dna_replication

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DNA Replication: A Closer Look * The copying of DNA is remarkable in its speed and accuracy * More than a dozen enzymes and other proteins participate in DNA replication Getting Started * Replication begins at particular sites called origins of replication, where the two DNA strands are separated, opening up a replication ?bubble? * A eukaryotic chromosome may have hundreds or even thousands of origins of replication * Replication proceeds in both directions from each origin, until the entire molecule is copied * At the end of each replication bubble is a replication fork, a Y-shaped region where new DNA strands are elongating * Helicases are enzymes that untwist the double helix at the replication forks * Single-strand binding proteins bind to and stabilize single-stranded DNA

Molecular Bionumbers Notes

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Adenosine triphosphate

Useful fundamental numbers in molecular biology The numbers quoted here were extracted from the literature. They should only serve as ?rule of thumb? values. Consult the full references to learn about the specific system under study, growth conditions, measurement method etc. Full references at: www.bioNumbers.org Cell sizes: Bacteria (e.coli): ~1mm diameter, 2mm length, ~1mm3 in volume; 109cells/ml in an overnight culture (OD600~1) Yeast (Saccharomyces cerevisiae): ~5 mm diameter, ~50 mm3 in volume Mammalian (HELA) cell - ~2,000 mm3 in volume, adherent cell on a slide ~20 mm diameter ? ~100,000 cells in a confluent well of a 96 multiwell plate Organelles and cell constituents: Mammalian cell nucleus ~10 micron diameter

Pearson Biology Guided Reading Answers Chapter 10

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DNA replication

Chapter 10: Molecular Biology of the Gene # 152826 Cust: Pearson Au: Reece Pg. No. 66 Title: Active Reading Guide for Campbell Biology: Concepts & Connections, 8e C / M / Y / K Short / Normal DESIGN SERVICES OF S4-CARLISLE Publishing Services 66 Copyright ? 2015 Pearson Education, Inc. Chapter 10: Molecular Biology of the Gene Guided Reading Activities Big idea: The structure of the genetic material Answer the following questions as you read modules 10.1?10.3: 1. The study of heredity at the molecular level is called ________________. 2. Students are usually surprised to discover that viruses can infect bacteria. What type of virus infects bacteria? 3. Hershey and Chase decided to use radioactively labeled sulfur and phosphorous in their

Pearson Biology Guided Reading Chapter 10

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Chapter 10: Molecular Biology of the Gene # 152826 Cust: Pearson Au: Reece Pg. No. 66 Title: Active Reading Guide for Campbell Biology: Concepts & Connections, 8e C / M / Y / K Short / Normal DESIGN SERVICES OF S4-CARLISLE Publishing Services 66 Copyright ? 2015 Pearson Education, Inc. Chapter 10: Molecular Biology of the Gene Guided Reading Activities Big idea: The structure of the genetic material Answer the following questions as you read modules 10.1?10.3: 1. The study of heredity at the molecular level is called ________________. 2. Students are usually surprised to discover that viruses can infect bacteria. What type of virus infects bacteria? 3. Hershey and Chase decided to use radioactively labeled sulfur and phosphorous in their

Pearson Guided Reading Activities KEY CH10

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Chapter 10: Molecular Biology of the Gene # 152826 Cust: Pearson Au: Reece Pg. No. 66 Title: Active Reading Guide for Campbell Biology: Concepts & Connections, 8e C / M / Y / K Short / Normal DESIGN SERVICES OF S4-CARLISLE Publishing Services 66 Copyright ? 2015 Pearson Education, Inc. Chapter 10: Molecular Biology of the Gene Guided Reading Activities Big idea: The structure of the genetic material Answer the following questions as you read modules 10.1?10.3: 1. The study of heredity at the molecular level is called ________________. 2. Students are usually surprised to discover that viruses can infect bacteria. What type of virus infects bacteria? 3. Hershey and Chase decided to use radioactively labeled sulfur and phosphorous in their

DNA Replication II

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Nick

DNA Replication a strand of DNA serves as a template for replication: complementary strain Meselson & Stahl use density centrifugation to determine method of replication grew bacterial in two different weights of Nitrogen media so the nitrogen bases have different weights switched media ruled out conservative replication need to separate strands to determine determined semi-conservative method is the true method replication begins at origin of replication bacterial and yeast: typically begin at high concentration of A/T point because there are only two hydrogen bonds between Adenine and Thymine whereas Guanine and Cytosine have three hydrogen bonds between them origin is 100-150 bp long 11-nucleotide core sequence of adenine and thymine and flanking adenine and thymine regions

DNA Replication I

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DNA Replication As the genetic information, DNA must be faithfully replicated and passed down to the next generation The structure of DNA immediately suggested a replication mechanism A strand of DNA can serve as a template for replication If you know one strand, you can determine the other What is the mechanism of replication? Meselson & Stahl used density centrifugation biomolecules will float or sink until they reach the point at which their density equals that of the gradient Meselson and Stahl?s results did not support the conservative replication model Using the same approach as Meselson and Stahl, how could you distinguish between these models? DNA replication initiates at Origins of Replication

Ch 16 AP BIOLOGY Study Guide from Biology Junction

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Chapter 16 The Molecular Basis of Inheritance Lecture Outline Overview: Life?s Operating Instructions In April 1953, James Watson and Francis Crick shook the scientific world with an elegant double-helical model for the structure of deoxyribonucleic acid, or DNA. Your genetic endowment is the DNA you inherited from your parents. Nucleic acids are unique in their ability to direct their own replication. The resemblance of offspring to their parents depends on the precise replication of DNA and its transmission from one generation to the next. It is this DNA program that directs the development of your biochemical, anatomical, physiological, and (to some extent) behavioral traits. Concept 16.1 DNA is the genetic material The search for genetic material led to DNA.
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