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Eukaryotic DNA replication

AP Bio_dna_replication

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DNA Replication: A Closer Look * The copying of DNA is remarkable in its speed and accuracy * More than a dozen enzymes and other proteins participate in DNA replication Getting Started * Replication begins at particular sites called origins of replication, where the two DNA strands are separated, opening up a replication ?bubble? * A eukaryotic chromosome may have hundreds or even thousands of origins of replication * Replication proceeds in both directions from each origin, until the entire molecule is copied * At the end of each replication bubble is a replication fork, a Y-shaped region where new DNA strands are elongating * Helicases are enzymes that untwist the double helix at the replication forks * Single-strand binding proteins bind to and stabilize single-stranded DNA

DNA Replication II

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DNA Replication a strand of DNA serves as a template for replication: complementary strain Meselson & Stahl use density centrifugation to determine method of replication grew bacterial in two different weights of Nitrogen media so the nitrogen bases have different weights switched media ruled out conservative replication need to separate strands to determine determined semi-conservative method is the true method replication begins at origin of replication bacterial and yeast: typically begin at high concentration of A/T point because there are only two hydrogen bonds between Adenine and Thymine whereas Guanine and Cytosine have three hydrogen bonds between them origin is 100-150 bp long 11-nucleotide core sequence of adenine and thymine and flanking adenine and thymine regions

DNA Replication I

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DNA Replication As the genetic information, DNA must be faithfully replicated and passed down to the next generation The structure of DNA immediately suggested a replication mechanism A strand of DNA can serve as a template for replication If you know one strand, you can determine the other What is the mechanism of replication? Meselson & Stahl used density centrifugation biomolecules will float or sink until they reach the point at which their density equals that of the gradient Meselson and Stahl?s results did not support the conservative replication model Using the same approach as Meselson and Stahl, how could you distinguish between these models? DNA replication initiates at Origins of Replication
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