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Pigment

ap bio lab 4

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Katie Hallstead Mrs. Legary AP Biology p.0 January 15, 2013 Lab 4: Plant Pigment and Photosynthesis Abstract In this experiment, there are two activities that aim to find two different things. In the first one, paper chromatography is used to separate the pigments found in chlorophyll. The second activity consists of measuring the rate of photosynthesis by observing the change in color that happens when DPIP is reduced through a time interval. Problem How many pigments exist within plants, and at what rates do they photosynthesize when exposed to different lights? Hypothesis

Lab #4 Plant Pigments and Photosynthesis - Questions

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Lab #4 Plant Pigments and Photosynthesis Exercise 4A: Plant Pigment Chromatography Topics of Discussion 1) What factors are involved in the separation of the pigments? 2) Would you expect the Rf value of a pigment to be the same if a different solvent were used? Explain. 3) What type of chlorophyll does the reaction center contain? What are the roles of the other pigments? Exercise 4B: Photosynthesis / The Light Reaction Topics of Discussion 1) What is the function of DPIP in this experiment? 2) What molecule found in chloroplasts does DPIP ?replace? in this experiment? 3) What is the source of the electrons that will reduce DPIP? 4) What was measured with the spectrophotometer in this experiment? 5) What is the effect of darkness on the reduction of DPIP? Explain.
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